TY - JOUR
T1 - Radiotolerance of N-cycle bacteria and their transcriptomic response to low-dose space-analogue ionizing irradiation
AU - Verbeelen, Tom
AU - Fernandez, Celia Alvarez
AU - Nguyen, Thanh Huy
AU - Gupta, Surya
AU - Leroy, Baptiste
AU - Wattiez, Ruddy
AU - Vlaeminck, Siegfried E.
AU - Leys, Natalie
AU - Ganigué, Ramon
AU - Mastroleo, Felice
N1 - Score=10
Publisher Copyright:
© 2024 The Author(s)
PY - 2024/5
Y1 - 2024/5
N2 - The advancement of regenerative life support systems (RLSS) is crucial to allow long-distance space travel. Within the Micro-Ecological Life Support System Alternative (MELiSSA), efficient nitrogen recovery from urine and other waste streams is vital to produce liquid fertilizer to feed food and oxygen production in subsequent photoautotrophic processes. This study explores the effects of ionizing radiation on nitrogen cycle bacteria that transform urea to nitrate. In particular, we assess the radiotolerance of Comamonas testosteroni, Nitrosomonas europaea, and Nitrobacter winogradskyi after exposure to acute γ-irradiation. Moreover, a comprehensive whole transcriptome analysis elucidates the effects of spaceflight-analogue low-dose ionizing radiation on the individual axenic strains and on their synthetic community o. This research sheds light on how the spaceflight environment could affect ureolysis and nitrification processes from a transcriptomic perspective.
AB - The advancement of regenerative life support systems (RLSS) is crucial to allow long-distance space travel. Within the Micro-Ecological Life Support System Alternative (MELiSSA), efficient nitrogen recovery from urine and other waste streams is vital to produce liquid fertilizer to feed food and oxygen production in subsequent photoautotrophic processes. This study explores the effects of ionizing radiation on nitrogen cycle bacteria that transform urea to nitrate. In particular, we assess the radiotolerance of Comamonas testosteroni, Nitrosomonas europaea, and Nitrobacter winogradskyi after exposure to acute γ-irradiation. Moreover, a comprehensive whole transcriptome analysis elucidates the effects of spaceflight-analogue low-dose ionizing radiation on the individual axenic strains and on their synthetic community o. This research sheds light on how the spaceflight environment could affect ureolysis and nitrification processes from a transcriptomic perspective.
KW - Microbiology
KW - Space sciences
UR - https://www.scopus.com/pages/publications/85189894329
U2 - 10.1016/j.isci.2024.109596
DO - 10.1016/j.isci.2024.109596
M3 - Article
AN - SCOPUS:85189894329
SN - 2589-0042
VL - 27
JO - iScience
JF - iScience
IS - 5
M1 - 109596
ER -