Abstract
A rapid and sensitive method for the detection of DNA in CsCl gradients is described, which is based on the complex formation between DNA and the intercalating dye ethidium bromide after fractionation of density gradients. Since no dye need be used in the original sample/CsCl mixture disadvantages such as dialysis, shaking with n-butanol, and/or running through a Dowex column can be avoided. Due to its sensitivity for nucleic acids (0.022 μg for DNA and 0.10 μg for RNA) this technique is most useful for small amounts of DNA, which are difficult to detect with a normal recording system or if no recording system is available at all.
| Original language | English |
|---|---|
| Pages (from-to) | 1-5 |
| Number of pages | 5 |
| Journal | Analytical Biochemistry |
| Volume | 108 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1980 |
Funding
We wish to thank Dr. P. Charles for the fractionation of the CsCl gradient with standard DNA species and providing us with labeled DNA, and Mrs. Jeannine Romeyer for typewriting the text. Work was supported by the European Communities.
| Funders | Funder number |
|---|---|
| 7th Framework Program of the European Atomic Energy Communities |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology