Regulation of the neutral phosphatase in Chlamydomonas reinhardi: An immunogenetic study of wild-type and mutant strains

R. Loppes; J. Braipson; R. F. Matagne; A. Sassen; L. Ledoux

doi:10.1007/BF00484505

Regulation of the neutral phosphatase in Chlamydomonas reinhardi: An immunogenetic study of wild-type and mutant strains

R. Loppes, J. Braipson, R. F. Matagne, A. Sassen, L. Ledoux

Research output › peer-review

Abstract

In Chlamydomonas reinhardi, the activity of the neutral phosphatase considerably increases when the cells are grown in the absence of inorganic phosphate (P_i). A comparative immunological study of cells grown on media containing P_i or not indicated that the neutral phosphatase was synthesized de novo. Ten mutants lacking the neutral phosphatase and distributed among three genetic loci (PD₂, PD₃, PD₂₄) were investigated for their ability to produce cross-reacting material (CRM) antigenically related to the wild enzyme. All mutants were shown to form much less CRM than the wild-type strain. It is proposed that the three genes are involved in the regulation of neutral phosphatase synthesis.

Original language	English
Pages (from-to)	1147-1157
Number of pages	11
Journal	Biochemical Genetics
Volume	15
Issue number	11-12
DOIs	https://doi.org/10.1007/BF00484505
State	Published - Dec 1977

ASJC Scopus subject areas

Biochemistry
Ecology, Evolution, Behavior and Systematics
Molecular Biology
Genetics

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10.1007/BF00484505

Cite this

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title = "Regulation of the neutral phosphatase in Chlamydomonas reinhardi: An immunogenetic study of wild-type and mutant strains",

abstract = "In Chlamydomonas reinhardi, the activity of the neutral phosphatase considerably increases when the cells are grown in the absence of inorganic phosphate (Pi). A comparative immunological study of cells grown on media containing Pi or not indicated that the neutral phosphatase was synthesized de novo. Ten mutants lacking the neutral phosphatase and distributed among three genetic loci (PD2, PD3, PD24) were investigated for their ability to produce cross-reacting material (CRM) antigenically related to the wild enzyme. All mutants were shown to form much less CRM than the wild-type strain. It is proposed that the three genes are involved in the regulation of neutral phosphatase synthesis.",

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AU - Loppes, R.

AU - Braipson, J.

AU - Matagne, R. F.

AU - Sassen, A.

AU - Ledoux, L.

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AB - In Chlamydomonas reinhardi, the activity of the neutral phosphatase considerably increases when the cells are grown in the absence of inorganic phosphate (Pi). A comparative immunological study of cells grown on media containing Pi or not indicated that the neutral phosphatase was synthesized de novo. Ten mutants lacking the neutral phosphatase and distributed among three genetic loci (PD2, PD3, PD24) were investigated for their ability to produce cross-reacting material (CRM) antigenically related to the wild enzyme. All mutants were shown to form much less CRM than the wild-type strain. It is proposed that the three genes are involved in the regulation of neutral phosphatase synthesis.

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