Abstract
The bromodeoxyuridine-Giemsa technique has been used to study systematically the incidence of cells in first or subsequent mitoses at different fixation times of human lymphocyte control cultures as well as influence of ionizing radiations on cell kinetics. Second divisions appear (3%) in cultures harvested 48 h after initiation. In 72 h cultures 40% of the dividing cells are in second and 33% in third division. Administration of 200 rads of X-rays before PHA stimulation results in a mitotic delay but does not increase the incidence of SCE. The yield of dicentrics after an exposure to 200 rads was the same for all cells in first mitosis regardless of fixation time. These results demonstrate that there is no evidence for the existence of sensitive subpopulations that could be distinguished by the time of the first mitotic division following stimulation.
Original language | English |
---|---|
Pages (from-to) | 473-478 |
Number of pages | 6 |
Journal | Canadian Journal of Genetics and Cytology |
Volume | 21 |
Issue number | 4 |
DOIs | |
State | Published - 1979 |
ASJC Scopus subject areas
- Genetics
- Plant Science
- Cell Biology