Reutilization of bacterial DNA by Arabidopsis thaliana cells in tissue culture

P. F. Lurquin; Y. Hotta

doi:10.1016/0304-4211(75)90050-4

Reutilization of bacterial DNA by Arabidopsis thaliana cells in tissue culture

P. F. Lurquin, Y. Hotta

Research output › peer-review

Abstract

Radiolabeled DNA purified from either Escherichia coli or Micrococcus lysodeikticus was supplied to callus cells of Arabidopsis thaliana grown on synthetic medium. After 7 days of incubation at 24°, total DNA was isolated from these cells and analyzed for the presence of bacterial DNA sequences. Isopycnic centrifugation of the preparations showed only A. thaliana DNA. Its reassociation properties were analyzed in the presence of excess unlabeled bacterial DNA, but no bacterial DNA sequences were detected. These results strongly indicate that if bacterial DNA sequences are integrated into the genome of Arabidopsis callus tissue, they must constitute less than 2-4% of the absorbed bacterial DNA. Varying the hormonal content of the culture medium did not affect this result.

Original language	English
Pages (from-to)	103-112
Number of pages	10
Journal	Plant Science Letters
Volume	5
Issue number	2
DOIs	https://doi.org/10.1016/0304-4211(75)90050-4
State	Published - Aug 1975

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10.1016/0304-4211(75)90050-4

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title = "Reutilization of bacterial DNA by Arabidopsis thaliana cells in tissue culture",

abstract = "Radiolabeled DNA purified from either Escherichia coli or Micrococcus lysodeikticus was supplied to callus cells of Arabidopsis thaliana grown on synthetic medium. After 7 days of incubation at 24°, total DNA was isolated from these cells and analyzed for the presence of bacterial DNA sequences. Isopycnic centrifugation of the preparations showed only A. thaliana DNA. Its reassociation properties were analyzed in the presence of excess unlabeled bacterial DNA, but no bacterial DNA sequences were detected. These results strongly indicate that if bacterial DNA sequences are integrated into the genome of Arabidopsis callus tissue, they must constitute less than 2-4% of the absorbed bacterial DNA. Varying the hormonal content of the culture medium did not affect this result.",

author = "Lurquin, {P. F.} and Y. Hotta",

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AU - Lurquin, P. F.

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N2 - Radiolabeled DNA purified from either Escherichia coli or Micrococcus lysodeikticus was supplied to callus cells of Arabidopsis thaliana grown on synthetic medium. After 7 days of incubation at 24°, total DNA was isolated from these cells and analyzed for the presence of bacterial DNA sequences. Isopycnic centrifugation of the preparations showed only A. thaliana DNA. Its reassociation properties were analyzed in the presence of excess unlabeled bacterial DNA, but no bacterial DNA sequences were detected. These results strongly indicate that if bacterial DNA sequences are integrated into the genome of Arabidopsis callus tissue, they must constitute less than 2-4% of the absorbed bacterial DNA. Varying the hormonal content of the culture medium did not affect this result.

AB - Radiolabeled DNA purified from either Escherichia coli or Micrococcus lysodeikticus was supplied to callus cells of Arabidopsis thaliana grown on synthetic medium. After 7 days of incubation at 24°, total DNA was isolated from these cells and analyzed for the presence of bacterial DNA sequences. Isopycnic centrifugation of the preparations showed only A. thaliana DNA. Its reassociation properties were analyzed in the presence of excess unlabeled bacterial DNA, but no bacterial DNA sequences were detected. These results strongly indicate that if bacterial DNA sequences are integrated into the genome of Arabidopsis callus tissue, they must constitute less than 2-4% of the absorbed bacterial DNA. Varying the hormonal content of the culture medium did not affect this result.

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