Separation of 90Nb from zirconium target for application in immuno-pet

Valery I. Radchenko, Dmitry V. Filosofov, O. K. Bochko, N. A. Lebedev, Alimardon V. Rakhimov, Harald Hauser, Michael Eisenhut, Nikolay V. Aksenov, Gospodin A. Bozhikov, Bernard Ponsard, Frank Roesch

    Research outputpeer-review

    22 Scopus citations


    Fast progressing immuno-PET asks to explore new radionuclides. One of the promising candidates is 90 Nb. It has a half-life of 14.6 h that allows visualizing and quantifying biological processes with medium and slow kinetics, such as tumor accumulation of antibodies and antibodies fragments or drug delivery systems and nanoparticles. 90 Nb exhibits a positron branching of 53% and an average kinetic energy of emitted positrons of E mean = 0.35MeV. Currently, radionuclide production routes and Nb V labeling techniques are explored to turn this radionuclide into a useful imaging probe.However, efficient separation of 90Nb from irradiated targets remains in challenge. Ion exchange based separation of 90Nb from zirconium targets was investigated in systems AG 1 × 8 - HCl/H2O2 and UTEVA-HCl. 95Nb (t 1/2 = 35.0 d), 95Zr ( 1/2 = 64.0 d) and 92mNb (t 1/2 = 10.15 d) were chosen for studies on distribution coefficients. Separation after AG 1 × 8 anion exchange yields 99% of 90/95 Nb. Subsequent use of a solidphase extraction step on UTEVA resin further decontaminates 90/95 Nb from traces of zirconium with yields 95% of 90/95Nb. A semi-automated separation takes one hour to obtain an overall recovery of 90/95Nb of 90%. The amount of Zr was reduced by factor of 108. The selected separation provides rapid preparation (< 1h) of high purity 90Nb appropriate for the synthesis of 90 Nb-radiopharmaceuticals, relevant for purposes of immuno-PET. Applying the radioniobium obtained, 90/95 Nb-labeling of a monoclonal antibody (rituximab) modified with desferrioxamine achieved labeling yields of> 90%after 1 h incubation at room temperature.

    Original languageEnglish
    Pages (from-to)433-442
    Number of pages10
    JournalRadiochimica Acta
    Issue number5
    StatePublished - May 2014

    ASJC Scopus subject areas

    • Physical and Theoretical Chemistry

    Cite this