TY - JOUR
T1 - Simulated microgravity decreases apoptosis in fetal fibroblasts
AU - Beck, Michaël
AU - Tabury, Kevin
AU - Moreels, Marjan
AU - Jacquet, Paul
AU - Van Oostveldt, Patrick
AU - De Vos, Winnok H.
AU - Baatout, Sarah
N1 - Score = 10
PY - 2012/2/20
Y1 - 2012/2/20
N2 - Space travel is a major challenge for human beings. Especially, the mechanisms through which space conditions might alter animal development have been questioned for a long time. The two major physical stress factors that are of relevance in this context are space radiation and weightlessness. While it has been extensively shown that high doses of ionizing radiation induce deleterious effects on embryonic development, so far, little is known about the potential harmful effects of radiation in combination with microgravity on the developing organism. In the present study, we investigated the effects of simulated microgravity on irradiated STO mouse fetal fibroblast cells using a random positioning machine (RPM). Radiation-induced cell cycle changes were not affected when cells were subjected to simulated microgravity for 24 h. Moreover, no morphological differences were observed in irradiated samples exposed to simulated microgravity compared to cells that were exclusively irradiated. However, microgravity simulation significantly decreased the level of apoptosis at all doses as measured by caspase-3 activity and it prevented cells from undergoing radiation-induced size increase up to 1 Gy.
AB - Space travel is a major challenge for human beings. Especially, the mechanisms through which space conditions might alter animal development have been questioned for a long time. The two major physical stress factors that are of relevance in this context are space radiation and weightlessness. While it has been extensively shown that high doses of ionizing radiation induce deleterious effects on embryonic development, so far, little is known about the potential harmful effects of radiation in combination with microgravity on the developing organism. In the present study, we investigated the effects of simulated microgravity on irradiated STO mouse fetal fibroblast cells using a random positioning machine (RPM). Radiation-induced cell cycle changes were not affected when cells were subjected to simulated microgravity for 24 h. Moreover, no morphological differences were observed in irradiated samples exposed to simulated microgravity compared to cells that were exclusively irradiated. However, microgravity simulation significantly decreased the level of apoptosis at all doses as measured by caspase-3 activity and it prevented cells from undergoing radiation-induced size increase up to 1 Gy.
KW - X-rays
KW - simulated microgravity
KW - fetal mouse fibroblasts
KW - DNA damage
KW - cell cycle arrest
KW - apoptosis
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_122675
UR - http://knowledgecentre.sckcen.be/so2/bibref/9325
U2 - 10.3892/ijmm.2012.1001
DO - 10.3892/ijmm.2012.1001
M3 - Article
VL - 30
SP - 309
EP - 313
JO - International Journal of Molecular Medicine
JF - International Journal of Molecular Medicine
IS - 2
ER -