Snow Surface Microbiome on the High Antarctic Plateau (DOME C)

Luigi Michaud, Angelina Giudice, Mohamed Ahmed, Pieter Monsieurs, Carmela Raffa, Natalie Leys, Stefano Amalfitano, Rob Van Houdt

    Research outputpeer-review


    The cryosphere is an integral part of the global climate system and one of the major habitable ecosystems of Earth's biosphere. These permanently frozen environments harbor diverse, viable and metabolically active microbial populations that represent almost all the major phylogenetic groups. In this study, we investigated the microbial diversity in the surface snow surrounding the Concordia Research Station on the High Antarctic Plateau through a polyphasic approach, including direct prokaryotic quantification by flow cytometry and catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH), and phylogenetic identification by 16S RNA gene clone library sequencing and 454 16S amplicon pyrosequencing. Although the microbial abundance was low (
    Original languageEnglish
    Pages (from-to)na-na
    JournalPLOS ONE
    Issue number8
    StatePublished - 7 Aug 2014

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