SPECT Imaging of Joint Inflammation with Nanobodies Targeting the Macrophage Mannose Receptor in a Mouse Model for Rheumatoid Arthritis

Stéphanie Put; Steve Schoonooghe; Nick Devoogdt; Evelien Schurgers; Anneleen Avau; Tania Mitera; Matthias D'huyvetter; Patrick De Baetselier; Geert Raes; Tony Lahoutte; Patrick Matthys; Sarah Baatout; An Aerts; Nathalie Impens

doi:10.2967/jnumed.112.111781

SPECT Imaging of Joint Inflammation with Nanobodies Targeting the Macrophage Mannose Receptor in a Mouse Model for Rheumatoid Arthritis

Stéphanie Put, Steve Schoonooghe, Nick Devoogdt, Evelien Schurgers, Anneleen Avau, Tania Mitera, Matthias D'huyvetter, Patrick De Baetselier, Geert Raes, Tony Lahoutte, Patrick Matthys, Sarah Baatout, An Aerts, Nathalie Impens

Research output › peer-review

Abstract

Rheumatoid arthritis (RA) is a chronic autoimmune disease occurring in approximately 1% of the worldwide population. The disease primarily affects the joints, where inflammatory cells, such as macrophages, invade the synovium and cause cartilage and bone destruction. Currently, it is difficult to efficiently diagnose and monitor early-stage RA. In this study, we investigated whether SPECT/micro-CT imaging with 99mTclabeled Nanobodies directed against the macrophage mannose receptor (MMR) is a useful tool for monitoring and quantifying joint inflammation in collagen-induced arthritis (CIA), a mouse model for RA. The expression of MMR was analyzed on macrophages and osteoclasts generated in vitro and in cells obtained from various organs from mice with CIA.

Original language	English
Pages (from-to)	807-814
Journal	Journal of Nuclear Medicine
Volume	54
Issue number	5
DOIs	https://doi.org/10.2967/jnumed.112.111781
State	Published - 27 Feb 2013

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Put, S., Schoonooghe, S., Devoogdt, N., Schurgers, E., Avau, A., Mitera, T., D'huyvetter, M., De Baetselier, P., Raes, G., Lahoutte, T., Matthys, P., Baatout, S., Aerts, A., & Impens, N. (2013). SPECT Imaging of Joint Inflammation with Nanobodies Targeting the Macrophage Mannose Receptor in a Mouse Model for Rheumatoid Arthritis. Journal of Nuclear Medicine, 54(5), 807-814. https://doi.org/10.2967/jnumed.112.111781

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title = "SPECT Imaging of Joint Inflammation with Nanobodies Targeting the Macrophage Mannose Receptor in a Mouse Model for Rheumatoid Arthritis",

abstract = "Rheumatoid arthritis (RA) is a chronic autoimmune disease occurring in approximately 1% of the worldwide population. The disease primarily affects the joints, where inflammatory cells, such as macrophages, invade the synovium and cause cartilage and bone destruction. Currently, it is difficult to efficiently diagnose and monitor early-stage RA. In this study, we investigated whether SPECT/micro-CT imaging with 99mTclabeled Nanobodies directed against the macrophage mannose receptor (MMR) is a useful tool for monitoring and quantifying joint inflammation in collagen-induced arthritis (CIA), a mouse model for RA. The expression of MMR was analyzed on macrophages and osteoclasts generated in vitro and in cells obtained from various organs from mice with CIA.",

keywords = "Rheumatoid Arthritis, Nanobody, Mannose Receptor, SPECT imaging",

author = "St{\'e}phanie Put and Steve Schoonooghe and Nick Devoogdt and Evelien Schurgers and Anneleen Avau and Tania Mitera and Matthias D'huyvetter and {De Baetselier}, Patrick and Geert Raes and Tony Lahoutte and Patrick Matthys and Sarah Baatout and An Aerts and Nathalie Impens",

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Put, S, Schoonooghe, S, Devoogdt, N, Schurgers, E, Avau, A, Mitera, T, D'huyvetter, M, De Baetselier, P, Raes, G, Lahoutte, T, Matthys, P, Baatout, S, Aerts, A & Impens, N 2013, 'SPECT Imaging of Joint Inflammation with Nanobodies Targeting the Macrophage Mannose Receptor in a Mouse Model for Rheumatoid Arthritis', Journal of Nuclear Medicine, vol. 54, no. 5, pp. 807-814. https://doi.org/10.2967/jnumed.112.111781

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AU - Put, Stéphanie

AU - Schoonooghe, Steve

AU - Devoogdt, Nick

AU - Schurgers, Evelien

AU - Avau, Anneleen

AU - Mitera, Tania

AU - D'huyvetter, Matthias

AU - De Baetselier, Patrick

AU - Raes, Geert

AU - Lahoutte, Tony

AU - Matthys, Patrick

A2 - Baatout, Sarah

A2 - Aerts, An

A2 - Impens, Nathalie

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N2 - Rheumatoid arthritis (RA) is a chronic autoimmune disease occurring in approximately 1% of the worldwide population. The disease primarily affects the joints, where inflammatory cells, such as macrophages, invade the synovium and cause cartilage and bone destruction. Currently, it is difficult to efficiently diagnose and monitor early-stage RA. In this study, we investigated whether SPECT/micro-CT imaging with 99mTclabeled Nanobodies directed against the macrophage mannose receptor (MMR) is a useful tool for monitoring and quantifying joint inflammation in collagen-induced arthritis (CIA), a mouse model for RA. The expression of MMR was analyzed on macrophages and osteoclasts generated in vitro and in cells obtained from various organs from mice with CIA.

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KW - Rheumatoid Arthritis

KW - Nanobody

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