SPECT Imaging of Joint Inflammation with Nanobodies Targeting the Macrophage Mannose Receptor in a Mouse Model for Rheumatoid Arthritis

Stéphanie Put, Steve Schoonooghe, Nick Devoogdt, Evelien Schurgers, Anneleen Avau, Tania Mitera, Matthias D'huyvetter, Patrick De Baetselier, Geert Raes, Tony Lahoutte, Patrick Matthys, Sarah Baatout, An Aerts, Nathalie Impens

    Research outputpeer-review


    Rheumatoid arthritis (RA) is a chronic autoimmune disease occurring in approximately 1% of the worldwide population. The disease primarily affects the joints, where inflammatory cells, such as macrophages, invade the synovium and cause cartilage and bone destruction. Currently, it is difficult to efficiently diagnose and monitor early-stage RA. In this study, we investigated whether SPECT/micro-CT imaging with 99mTclabeled Nanobodies directed against the macrophage mannose receptor (MMR) is a useful tool for monitoring and quantifying joint inflammation in collagen-induced arthritis (CIA), a mouse model for RA. The expression of MMR was analyzed on macrophages and osteoclasts generated in vitro and in cells obtained from various organs from mice with CIA.
    Original languageEnglish
    Pages (from-to)807-814
    JournalJournal of Nuclear Medicine
    Issue number5
    StatePublished - 27 Feb 2013

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