Strontium-induced genomic responses of Cupriavidus metallidurans and strontium bioprecipitation as strontium carbonate

Issam Ben Salem; Haïtham Sghaier; Pieter Monsieurs; Hugo Moors; Rob Van Houdt; Sami Fattouch; Mouldi Saidi; Ahmed Landolsi; Natalie Leys

doi:10.1007/s13213-012-0462-3

Strontium-induced genomic responses of Cupriavidus metallidurans and strontium bioprecipitation as strontium carbonate

Issam Ben Salem, Haïtham Sghaier, Pieter Monsieurs, Hugo Moors, Rob Van Houdt, Sami Fattouch, Mouldi Saidi, Ahmed Landolsi, Natalie Leys

Research output › peer-review

3 Scopus citations

Abstract

The physiological and global transcriptomic response of the metal-resistant bacterium Cupriavidus metallidurans CH34 to stable non-radioactive strontium (Sr) was investigated. C. metallidurans CH34 was able to survive and proliferate in the presence of relatively high concentrations of SrCl₂ (concentration of Sr needed to inactivate 90 % of the cells = 70 mM; minimum inhibitory concentration = 120 mM). Gene expression in cells was analyzed after short-term (30 min) exposure to low (5 mM) and high (60 mM) concentrations of SrCl₂. The transcription of the gene clusters annotated as hmyFCBA and czcCBADRS, coding for ion efflux pumps, was significantly induced following exposure to Sr relative to that in non-stressed CH34 cells. Sr precipitation was observed during aerobic growth of CH34 in the presence of 60 mM SrCl ₂. Transmission electron microscopy images of cells grown with Sr also showed very small solid-phase precipitates located in the vicinity of cells, but mainly extracellular. Environmental scanning electron microscopy with energy-dispersive X-ray spectrometry indicated that the sequestered extracellular solid-phase Sr was present in the form of strontium carbonate.

Original language	English
Pages (from-to)	833-844
Number of pages	12
Journal	Annals of Microbiology
Volume	63
Issue number	3
DOIs	https://doi.org/10.1007/s13213-012-0462-3
State	Published - Sep 2013

ASJC Scopus subject areas

Applied Microbiology and Biotechnology

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10.1007/s13213-012-0462-3

Cite this

@article{bbb0c604c8b0467cbc0297b0e8df5f2a,

title = "Strontium-induced genomic responses of Cupriavidus metallidurans and strontium bioprecipitation as strontium carbonate",

abstract = "The physiological and global transcriptomic response of the metal-resistant bacterium Cupriavidus metallidurans CH34 to stable non-radioactive strontium (Sr) was investigated. C. metallidurans CH34 was able to survive and proliferate in the presence of relatively high concentrations of SrCl2 (concentration of Sr needed to inactivate 90 % of the cells = 70 mM; minimum inhibitory concentration = 120 mM). Gene expression in cells was analyzed after short-term (30 min) exposure to low (5 mM) and high (60 mM) concentrations of SrCl2. The transcription of the gene clusters annotated as hmyFCBA and czcCBADRS, coding for ion efflux pumps, was significantly induced following exposure to Sr relative to that in non-stressed CH34 cells. Sr precipitation was observed during aerobic growth of CH34 in the presence of 60 mM SrCl 2. Transmission electron microscopy images of cells grown with Sr also showed very small solid-phase precipitates located in the vicinity of cells, but mainly extracellular. Environmental scanning electron microscopy with energy-dispersive X-ray spectrometry indicated that the sequestered extracellular solid-phase Sr was present in the form of strontium carbonate.",

keywords = "Bioprecipitation, Cupriavidus metallidurans CH34, Efflux system, Microarray, RT-qPCR, Strontium",

author = "{Ben Salem}, Issam and Ha{\"i}tham Sghaier and Pieter Monsieurs and Hugo Moors and {Van Houdt}, Rob and Sami Fattouch and Mouldi Saidi and Ahmed Landolsi and Natalie Leys",

note = " {\textcopyright} 2012 Springer-Verlag and the University of Milan.",

year = "2013",

month = sep,

doi = "10.1007/s13213-012-0462-3",

language = "English",

volume = "63",

pages = "833--844",

journal = "Annals of Microbiology",

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T1 - Strontium-induced genomic responses of Cupriavidus metallidurans and strontium bioprecipitation as strontium carbonate

AU - Ben Salem, Issam

AU - Sghaier, Haïtham

AU - Monsieurs, Pieter

AU - Moors, Hugo

AU - Van Houdt, Rob

AU - Fattouch, Sami

AU - Saidi, Mouldi

AU - Landolsi, Ahmed

AU - Leys, Natalie

PY - 2013/9

Y1 - 2013/9

N2 - The physiological and global transcriptomic response of the metal-resistant bacterium Cupriavidus metallidurans CH34 to stable non-radioactive strontium (Sr) was investigated. C. metallidurans CH34 was able to survive and proliferate in the presence of relatively high concentrations of SrCl2 (concentration of Sr needed to inactivate 90 % of the cells = 70 mM; minimum inhibitory concentration = 120 mM). Gene expression in cells was analyzed after short-term (30 min) exposure to low (5 mM) and high (60 mM) concentrations of SrCl2. The transcription of the gene clusters annotated as hmyFCBA and czcCBADRS, coding for ion efflux pumps, was significantly induced following exposure to Sr relative to that in non-stressed CH34 cells. Sr precipitation was observed during aerobic growth of CH34 in the presence of 60 mM SrCl 2. Transmission electron microscopy images of cells grown with Sr also showed very small solid-phase precipitates located in the vicinity of cells, but mainly extracellular. Environmental scanning electron microscopy with energy-dispersive X-ray spectrometry indicated that the sequestered extracellular solid-phase Sr was present in the form of strontium carbonate.

AB - The physiological and global transcriptomic response of the metal-resistant bacterium Cupriavidus metallidurans CH34 to stable non-radioactive strontium (Sr) was investigated. C. metallidurans CH34 was able to survive and proliferate in the presence of relatively high concentrations of SrCl2 (concentration of Sr needed to inactivate 90 % of the cells = 70 mM; minimum inhibitory concentration = 120 mM). Gene expression in cells was analyzed after short-term (30 min) exposure to low (5 mM) and high (60 mM) concentrations of SrCl2. The transcription of the gene clusters annotated as hmyFCBA and czcCBADRS, coding for ion efflux pumps, was significantly induced following exposure to Sr relative to that in non-stressed CH34 cells. Sr precipitation was observed during aerobic growth of CH34 in the presence of 60 mM SrCl 2. Transmission electron microscopy images of cells grown with Sr also showed very small solid-phase precipitates located in the vicinity of cells, but mainly extracellular. Environmental scanning electron microscopy with energy-dispersive X-ray spectrometry indicated that the sequestered extracellular solid-phase Sr was present in the form of strontium carbonate.

KW - Bioprecipitation

KW - Cupriavidus metallidurans CH34

KW - Efflux system

KW - Microarray

KW - RT-qPCR

KW - Strontium

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