Studies on Agrobacterium tumefaciens IV. Nonreplication of the bacterial DNA in mung bean (Phaseolus aureus)

C. I. Kado; P. F. Lurquin

doi:10.1016/0006-291X(75)90235-1

Studies on Agrobacterium tumefaciens IV. Nonreplication of the bacterial DNA in mung bean (Phaseolus aureus)

C. I. Kado, P. F. Lurquin

Research output › peer-review

Abstract

DNA·DNA filter hybridization and DNA solution enrichment reassociation experiments showed that no Agrobacterium tumefaciens DNA was replicated in mung bean seedlings under the conditions specified in published reports for the uptake, integration, and replication of bacterial DNA in higher plants. Residual presumptive DNA hybrids that formed in a few instances were characterized by thermal chromatography on hydroxylapatite. The Tm and melting profiles of these hybrids from DNA-treated plants were the same as those from untreated control plants. The sensitivities of these procedures were sufficient to detect A. tumefaciens DNA in the order of 0.005% to 0.01% of the plant genome. These results do not concur with previous reports that large pieces of DNA (at least 30%) of the plant genome of bacterial-DNA-treated-plants is made up of bacterial donor DNA.

Original language	English
Pages (from-to)	175-183
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	64
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(75)90235-1
State	Published - 5 May 1975

Funding

Funders	Funder number
National Cancer Institute (NCI)

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0006-291X(75)90235-1

Cite this

@article{d2bceed822b948f8baf76286994f8395,

title = "Studies on Agrobacterium tumefaciens IV. Nonreplication of the bacterial DNA in mung bean (Phaseolus aureus)",

abstract = "DNA·DNA filter hybridization and DNA solution enrichment reassociation experiments showed that no Agrobacterium tumefaciens DNA was replicated in mung bean seedlings under the conditions specified in published reports for the uptake, integration, and replication of bacterial DNA in higher plants. Residual presumptive DNA hybrids that formed in a few instances were characterized by thermal chromatography on hydroxylapatite. The Tm and melting profiles of these hybrids from DNA-treated plants were the same as those from untreated control plants. The sensitivities of these procedures were sufficient to detect A. tumefaciens DNA in the order of 0.005\% to 0.01\% of the plant genome. These results do not concur with previous reports that large pieces of DNA (at least 30\%) of the plant genome of bacterial-DNA-treated-plants is made up of bacterial donor DNA.",

author = "Kado, \{C. I.\} and Lurquin, \{P. F.\}",

year = "1975",

month = may,

day = "5",

doi = "10.1016/0006-291X(75)90235-1",

language = "English",

volume = "64",

pages = "175--183",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Studies on Agrobacterium tumefaciens IV. Nonreplication of the bacterial DNA in mung bean (Phaseolus aureus)

AU - Kado, C. I.

AU - Lurquin, P. F.

PY - 1975/5/5

Y1 - 1975/5/5

N2 - DNA·DNA filter hybridization and DNA solution enrichment reassociation experiments showed that no Agrobacterium tumefaciens DNA was replicated in mung bean seedlings under the conditions specified in published reports for the uptake, integration, and replication of bacterial DNA in higher plants. Residual presumptive DNA hybrids that formed in a few instances were characterized by thermal chromatography on hydroxylapatite. The Tm and melting profiles of these hybrids from DNA-treated plants were the same as those from untreated control plants. The sensitivities of these procedures were sufficient to detect A. tumefaciens DNA in the order of 0.005% to 0.01% of the plant genome. These results do not concur with previous reports that large pieces of DNA (at least 30%) of the plant genome of bacterial-DNA-treated-plants is made up of bacterial donor DNA.

AB - DNA·DNA filter hybridization and DNA solution enrichment reassociation experiments showed that no Agrobacterium tumefaciens DNA was replicated in mung bean seedlings under the conditions specified in published reports for the uptake, integration, and replication of bacterial DNA in higher plants. Residual presumptive DNA hybrids that formed in a few instances were characterized by thermal chromatography on hydroxylapatite. The Tm and melting profiles of these hybrids from DNA-treated plants were the same as those from untreated control plants. The sensitivities of these procedures were sufficient to detect A. tumefaciens DNA in the order of 0.005% to 0.01% of the plant genome. These results do not concur with previous reports that large pieces of DNA (at least 30%) of the plant genome of bacterial-DNA-treated-plants is made up of bacterial donor DNA.

UR - https://www.scopus.com/pages/publications/0016757168

U2 - 10.1016/0006-291X(75)90235-1

DO - 10.1016/0006-291X(75)90235-1

M3 - Article

C2 - 1147920

AN - SCOPUS:0016757168

SN - 0006-291X

VL - 64

SP - 175

EP - 183

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -