TY - JOUR
T1 - Study of oxidative stress related responses induced in Arabidopsis thaliana following mixed exposure to uranium and cadmium
AU - Vanhoudt, Nathalie
AU - Vandenhove, Hildegarde
AU - Horemans, Nele
AU - Wannijn, Jean
AU - Bujanic, Andelko
AU - Vangronsveld, Jaco
AU - Cuypers, Ann
N1 - Score = 10
PY - 2010/8
Y1 - 2010/8
N2 - Toxicity effects in plants of uranium in a binary pollution condition were investigated by studying biological responses and unraveling oxidative stress related mechanisms in Arabidopsis thaliana seedlings, grown on hydroponics and exposed for 3 days to 10 µM uranium in combination with 5 µM cadmium. While uranium mostly accumulated in the roots with very low root-to-shoot transport, cadmium was taken up less by the roots but showed higher translocation to the shoots. Cadmium influenced uranium uptake highly but not the other way round resulting in a doubled uranium concentration in the roots. Under mixed exposure conditions, it is clear that micronutrient concentrations in the roots are strongly influenced by addition of cadmium as a second stressor, while leaf macronutrient concentrations are mostly influenced by uranium. Oxidative stress related responses are highly affected by cadmium while uranium influence is more limited. An important role was attributed to the ascorbate redox balance together with glutathione as both metabolites increased their reduced form, indicating an important defense and regulatory function. While for roots, based on an increase in FSD1 gene expression, oxidative stress was suggested to be superoxide induced, in leaves on the other hand, hydrogen peroxide related genes were mostly altered.
AB - Toxicity effects in plants of uranium in a binary pollution condition were investigated by studying biological responses and unraveling oxidative stress related mechanisms in Arabidopsis thaliana seedlings, grown on hydroponics and exposed for 3 days to 10 µM uranium in combination with 5 µM cadmium. While uranium mostly accumulated in the roots with very low root-to-shoot transport, cadmium was taken up less by the roots but showed higher translocation to the shoots. Cadmium influenced uranium uptake highly but not the other way round resulting in a doubled uranium concentration in the roots. Under mixed exposure conditions, it is clear that micronutrient concentrations in the roots are strongly influenced by addition of cadmium as a second stressor, while leaf macronutrient concentrations are mostly influenced by uranium. Oxidative stress related responses are highly affected by cadmium while uranium influence is more limited. An important role was attributed to the ascorbate redox balance together with glutathione as both metabolites increased their reduced form, indicating an important defense and regulatory function. While for roots, based on an increase in FSD1 gene expression, oxidative stress was suggested to be superoxide induced, in leaves on the other hand, hydrogen peroxide related genes were mostly altered.
KW - Arabidopsis thaliana
KW - binary mixture
KW - cadmium
KW - gene expression
KW - oxidative stress
KW - uranium
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_108796
UR - http://knowledgecentre.sckcen.be/so2/bibref/7291
U2 - 10.1016/j.plaphy.2010.08.005
DO - 10.1016/j.plaphy.2010.08.005
M3 - Article
SN - 0981-9428
VL - 48
SP - 879
EP - 886
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
IS - 10-11
ER -