Surface proteins of radiation-induced and radiation leukemia virus-induced thymic lymphosarcomas in mice

Robert J. Hooghe; Daniel C. Hoessli; Michel Janowski

doi:10.1016/0145-2126(83)90010-3

Surface proteins of radiation-induced and radiation leukemia virus-induced thymic lymphosarcomas in mice

Robert J. Hooghe, Daniel C. Hoessli, Michel Janowski

Research output › peer-review

Abstract

Thymic lymphosarcomas (TLS) were induced in C57BL mice by X-rays or by Radiation Leukemia Virus (RadLV) and their surface glycoproteins (gps) compared after cell-surface radio-iodination and polyacrylamide gel electrophoresis (SDS-PAGE). All lymphocytic antigens tested (T200, 170 100, Thy-1) and proteins with apparent molecular weight (Mr) around 120,000 and 100,000 were present on all tumours, as well as retrovirus - encoded proteins but considerable variation in the Mr of several serologically-related proteins was observed. Therefore, the TLS in C57BL mice form a heterogeneous group, suggesting that T cells can be transformed at different stages of maturation. The possibility that transformation allows or even triggers differentiation is also entertained.

Original language	English
Pages (from-to)	205-213,215-219
Journal	Leukemia Research
Volume	7
Issue number	2
DOIs	https://doi.org/10.1016/0145-2126(83)90010-3
State	Published - 1983

Funding

*Supported by grants from the Belgian Foods de la Recherche Scientifique M~dicale, the European Atomic Energy Commission and the European Late Effect Project. Abbreviations: B cell, bursa-derived lymphocyte; T cell, thymus-derived lymphocyte; BMI, CP3, NP1, RH1, T2, TL2, VL3 .... cell lines (listed in Table 2); gp, (cellular or viral) glycoprotein; HMW, high molecular weight; LN, lymph node; Mr, apparent molecular weight; RadLV, Radiation Leukemia Virus; RadLV/VL3, virus produced by the cell line BL/VL3; SDS-PAGE, polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate; Staph A, formalin-fixed Staphylococcus aureus, strain Cowan 1; thc, thymoc3tte; TLS, thymic lymphosarcoma. Correspondence to: Dr. Robert I-looghe, Radiobiology, S.C.K., Steenweg op Retie, B-2440 Geel, Belgium.

Funders	Funder number
EURATOM - European Atomic Energy Community

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

Access to Document

10.1016/0145-2126(83)90010-3

Cite this

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title = "Surface proteins of radiation-induced and radiation leukemia virus-induced thymic lymphosarcomas in mice",

abstract = "Thymic lymphosarcomas (TLS) were induced in C57BL mice by X-rays or by Radiation Leukemia Virus (RadLV) and their surface glycoproteins (gps) compared after cell-surface radio-iodination and polyacrylamide gel electrophoresis (SDS-PAGE). All lymphocytic antigens tested (T200, 170 100, Thy-1) and proteins with apparent molecular weight (Mr) around 120,000 and 100,000 were present on all tumours, as well as retrovirus - encoded proteins but considerable variation in the Mr of several serologically-related proteins was observed. Therefore, the TLS in C57BL mice form a heterogeneous group, suggesting that T cells can be transformed at different stages of maturation. The possibility that transformation allows or even triggers differentiation is also entertained.",

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T1 - Surface proteins of radiation-induced and radiation leukemia virus-induced thymic lymphosarcomas in mice

AU - Hooghe, Robert J.

AU - Hoessli, Daniel C.

AU - Janowski, Michel

PY - 1983

Y1 - 1983

N2 - Thymic lymphosarcomas (TLS) were induced in C57BL mice by X-rays or by Radiation Leukemia Virus (RadLV) and their surface glycoproteins (gps) compared after cell-surface radio-iodination and polyacrylamide gel electrophoresis (SDS-PAGE). All lymphocytic antigens tested (T200, 170 100, Thy-1) and proteins with apparent molecular weight (Mr) around 120,000 and 100,000 were present on all tumours, as well as retrovirus - encoded proteins but considerable variation in the Mr of several serologically-related proteins was observed. Therefore, the TLS in C57BL mice form a heterogeneous group, suggesting that T cells can be transformed at different stages of maturation. The possibility that transformation allows or even triggers differentiation is also entertained.

AB - Thymic lymphosarcomas (TLS) were induced in C57BL mice by X-rays or by Radiation Leukemia Virus (RadLV) and their surface glycoproteins (gps) compared after cell-surface radio-iodination and polyacrylamide gel electrophoresis (SDS-PAGE). All lymphocytic antigens tested (T200, 170 100, Thy-1) and proteins with apparent molecular weight (Mr) around 120,000 and 100,000 were present on all tumours, as well as retrovirus - encoded proteins but considerable variation in the Mr of several serologically-related proteins was observed. Therefore, the TLS in C57BL mice form a heterogeneous group, suggesting that T cells can be transformed at different stages of maturation. The possibility that transformation allows or even triggers differentiation is also entertained.

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KW - glycoprotein

KW - lectins

KW - membrane

KW - monoclonal antibody

KW - radiation

KW - retrovirus

KW - T lymphocyte

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