TY - JOUR
T1 - The cellular redox state as a modulator in cadmium and copper responses in Arabidopsis thaliana seedlings
AU - Cuypers, Ann
AU - Smeets, Karen
AU - Ruytinx, Jos
AU - Opdenakker, Kelly
AU - Keunen, Els
AU - Remans, Tony
AU - Horemans, Nele
AU - Vanhoudt, Nathalie
AU - Van sanden, Suzy
AU - Van Bellegem, Frank
AU - Guisez, Yves
AU - Colpaert, Jan
AU - Vangrondsveld, Jaco
A2 - Vandenhove, Hildegarde
A2 - Vanmarcke, Hans
N1 - Score = 10
PY - 2011/1
Y1 - 2011/1
N2 - The cellular redox state is an important determinant of metal phytotoxicity. Here the influence of cadmium (Cd) and copper (Cu) stress on the cellular redox balance in relation to oxidative signalling and damage in Arabidopsis thaliana is investigated . Both metals were easily taken up by the roots, but the translocation to the aboveground parts was restricted to Cd stress. In the roots, Cu directly induced an oxidative burst, whereas enzymatic ROS (reactive oxygen species) production via NADPH oxidases seems important in oxidative stress caused by Cd. Furthermore, in the roots, the glutathione metabolism plays a crucial role in controlling the gene regulation of the antioxidative defence mechanism under Cd stress.
Metal-specific alterations were also noticed with regard to the microRNA regulation of CuZnSOD gene expression in both roots and leaves.Lipid peroxidation could be an indication of oxidative damage as well as an indication of oxidative signalling as lipoxygenases are induced after metal exposure and are initial enzymes in oxylipin biosynthesis.
In conclusion, the metal-induced cellular redox imbalance is strongly dependent on the chemical properties of the metal and the plant organ considered. The stress intensity determines its involvement in downstream responses in relation to oxidative damage or signalling.
AB - The cellular redox state is an important determinant of metal phytotoxicity. Here the influence of cadmium (Cd) and copper (Cu) stress on the cellular redox balance in relation to oxidative signalling and damage in Arabidopsis thaliana is investigated . Both metals were easily taken up by the roots, but the translocation to the aboveground parts was restricted to Cd stress. In the roots, Cu directly induced an oxidative burst, whereas enzymatic ROS (reactive oxygen species) production via NADPH oxidases seems important in oxidative stress caused by Cd. Furthermore, in the roots, the glutathione metabolism plays a crucial role in controlling the gene regulation of the antioxidative defence mechanism under Cd stress.
Metal-specific alterations were also noticed with regard to the microRNA regulation of CuZnSOD gene expression in both roots and leaves.Lipid peroxidation could be an indication of oxidative damage as well as an indication of oxidative signalling as lipoxygenases are induced after metal exposure and are initial enzymes in oxylipin biosynthesis.
In conclusion, the metal-induced cellular redox imbalance is strongly dependent on the chemical properties of the metal and the plant organ considered. The stress intensity determines its involvement in downstream responses in relation to oxidative damage or signalling.
KW - Arabidopsis thaliana
KW - metal stress
KW - oxidative stress
KW - singal transduction
KW - cellular redox state
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_110273
UR - http://knowledgecentre.sckcen.be/so2/bibref/7870
U2 - 10.1016/j.jplph.2010.07.010
DO - 10.1016/j.jplph.2010.07.010
M3 - Article
SN - 0176-1617
VL - 168
SP - 309
EP - 316
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
IS - 4
ER -