The chromatography of nucleic acid preparations on deaecellulose paper. II. Interrelations of the fractions

C. Davila; P. Charles; L. Ledoux

doi:10.1016/s0021-9673(01)99475-0

The chromatography of nucleic acid preparations on deaecellulose paper. II. Interrelations of the fractions

C. Davila, P. Charles, L. Ledoux

Research output › peer-review

Abstract

The fractions obtained by chromatography of DNA preparations on DEAE-cellulose paper have been characterized by different means including dialysis, alcohol and acid precipitation, optical density measurements (hyperchromic effect). The fractionation procedure can be used to follow the effect of ageing (and of spontaneous radiolysis), ultrasonic vibrations, thermal denaturation and hydrolysis by DNAase. The concept "DNA prepration" is discussed with respect to the specific behaviour of the different fractions into which the original mixture can be resolved.

Original language	English
Pages (from-to)	396-403
Number of pages	8
Journal	Journal of Chromatography A
Volume	19
Issue number	C
DOIs	https://doi.org/10.1016/s0021-9673(01)99475-0
State	Published - 1965

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

Access to Document

10.1016/s0021-9673(01)99475-0

Cite this

@article{472405d04b2443188470a37fe53ba37c,

title = "The chromatography of nucleic acid preparations on deaecellulose paper. II. Interrelations of the fractions",

abstract = "The fractions obtained by chromatography of DNA preparations on DEAE-cellulose paper have been characterized by different means including dialysis, alcohol and acid precipitation, optical density measurements (hyperchromic effect). The fractionation procedure can be used to follow the effect of ageing (and of spontaneous radiolysis), ultrasonic vibrations, thermal denaturation and hydrolysis by DNAase. The concept {"}DNA prepration{"} is discussed with respect to the specific behaviour of the different fractions into which the original mixture can be resolved.",

author = "C. Davila and P. Charles and L. Ledoux",

year = "1965",

doi = "10.1016/s0021-9673(01)99475-0",

language = "English",

volume = "19",

pages = "396--403",

journal = "Journal of Chromatography A",

issn = "0021-9673",

publisher = "Elsevier B.V.",

number = "C",

}

TY - JOUR

T1 - The chromatography of nucleic acid preparations on deaecellulose paper. II. Interrelations of the fractions

AU - Davila, C.

AU - Charles, P.

AU - Ledoux, L.

PY - 1965

Y1 - 1965

N2 - The fractions obtained by chromatography of DNA preparations on DEAE-cellulose paper have been characterized by different means including dialysis, alcohol and acid precipitation, optical density measurements (hyperchromic effect). The fractionation procedure can be used to follow the effect of ageing (and of spontaneous radiolysis), ultrasonic vibrations, thermal denaturation and hydrolysis by DNAase. The concept "DNA prepration" is discussed with respect to the specific behaviour of the different fractions into which the original mixture can be resolved.

AB - The fractions obtained by chromatography of DNA preparations on DEAE-cellulose paper have been characterized by different means including dialysis, alcohol and acid precipitation, optical density measurements (hyperchromic effect). The fractionation procedure can be used to follow the effect of ageing (and of spontaneous radiolysis), ultrasonic vibrations, thermal denaturation and hydrolysis by DNAase. The concept "DNA prepration" is discussed with respect to the specific behaviour of the different fractions into which the original mixture can be resolved.

UR - http://www.scopus.com/inward/record.url?scp=0013793487&partnerID=8YFLogxK

U2 - 10.1016/s0021-9673(01)99475-0

DO - 10.1016/s0021-9673(01)99475-0

M3 - Article

C2 - 5323695

AN - SCOPUS:0013793487

SN - 0021-9673

VL - 19

SP - 396

EP - 403

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - C

ER -