TY - JOUR
T1 - The combined effect of uranium and gamma radiation on biological responses and oxidative stress induced in Arabidopsis thaliana
AU - Vanhoudt, Nathalie
AU - Vandenhove, Hildegarde
AU - Horemans, Nele
AU - Wannijn, Jean
AU - Van Hees, May
AU - Vangronsveld, Jaco
AU - Cuypers, Ann
N1 - Score = 10
PY - 2010/11
Y1 - 2010/11
N2 - Uranium never occurs as a single pollutant in the environment, but always in combination with other stressors such as ionizing radiation. As effects induced by multiple contaminants can differ markedly from the effects induced by the individual stressors, this multiple pollution context should not be neglected. In this study, effects on growth, nutrient uptake and oxidative stress induced by the single stressors uranium and gamma radiation are compared with the effects induced by the combination of both stressors. By doing this, we aim to better understand the effects induced by the combined stressors but also to get more insight in stressor-specific response mechanisms. Eighteen-day-old Arabidopsis thaliana seedlings were exposed for 3 days to 10 µM uranium and 3.5 Gy gamma radiation. Gamma radiation interfered with uranium uptake, resulting in decreased uranium concentrations in the roots, but with higher transport to the leaves. This resulted in a better root growth but increased leaf lipid peroxidation. For the other endpoints studied, effects under combined exposure were mostly determined by uranium presence and only limited influenced by gamma presence. Furthermore, an important role is suggested for CAT1/2/3 gene expression under uranium and mixed stressor conditions in the leaves.
AB - Uranium never occurs as a single pollutant in the environment, but always in combination with other stressors such as ionizing radiation. As effects induced by multiple contaminants can differ markedly from the effects induced by the individual stressors, this multiple pollution context should not be neglected. In this study, effects on growth, nutrient uptake and oxidative stress induced by the single stressors uranium and gamma radiation are compared with the effects induced by the combination of both stressors. By doing this, we aim to better understand the effects induced by the combined stressors but also to get more insight in stressor-specific response mechanisms. Eighteen-day-old Arabidopsis thaliana seedlings were exposed for 3 days to 10 µM uranium and 3.5 Gy gamma radiation. Gamma radiation interfered with uranium uptake, resulting in decreased uranium concentrations in the roots, but with higher transport to the leaves. This resulted in a better root growth but increased leaf lipid peroxidation. For the other endpoints studied, effects under combined exposure were mostly determined by uranium presence and only limited influenced by gamma presence. Furthermore, an important role is suggested for CAT1/2/3 gene expression under uranium and mixed stressor conditions in the leaves.
KW - Arabidopsis thaliana
KW - enzyme capacity
KW - gamma radiation
KW - gene expression
KW - oxidative stress
KW - uranium contamination
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_107176
UR - http://knowledgecentre.sckcen.be/so2/bibref/7188
U2 - 10.1016/j.jenvrad.2010.06.008
DO - 10.1016/j.jenvrad.2010.06.008
M3 - Article
VL - 101
SP - 923
EP - 930
JO - Journal of environmental radioactivity
JF - Journal of environmental radioactivity
IS - 11
ER -