TY - JOUR
T1 - Transcriptional response to ionizing radiation in lymphocyte subsets
AU - Mori, M.
AU - Benotmane, M. A.
AU - Tirone, I.
AU - Hooghe-Peters, E. L.
AU - Desaintes, C.
N1 - Score = 10
PY - 2005/7
Y1 - 2005/7
N2 - Human lymphocyte subpopulations differ in their cellular responses to ionizing radiation. To shed light on the molecular basis of this effect, we characterized the transcriptional response to 1 Gy X-rays of CD4+ T lymphocytes. Of 18,433 genes tested, 102 were modulated more than 1.5-fold. The majority of the strongly activated genes were p53 targets involved in DNA repair and apoptosis. The expression of three of these genes was further tested by quantitative RT-PCR in lymphocyte subpopulations [CD4+ and CD8+ T, CD19+ B, CD56+ natural killer cells and peripheral blood lymphocytes (PBLs)] from ten adult donors. In contrast to DDB2, TNFRSF10B and BAX were differentially modulated among the subpopulations and the PBLs, being more activated in irradiated CD19+ B and CD8+ T lymphocytes. The level of BAX activation in the various subpopulations correlated with the sensitivity of the cells to radiation, suggesting its possible role in the differential radiosensitivity of hematopoietic cell subsets.
AB - Human lymphocyte subpopulations differ in their cellular responses to ionizing radiation. To shed light on the molecular basis of this effect, we characterized the transcriptional response to 1 Gy X-rays of CD4+ T lymphocytes. Of 18,433 genes tested, 102 were modulated more than 1.5-fold. The majority of the strongly activated genes were p53 targets involved in DNA repair and apoptosis. The expression of three of these genes was further tested by quantitative RT-PCR in lymphocyte subpopulations [CD4+ and CD8+ T, CD19+ B, CD56+ natural killer cells and peripheral blood lymphocytes (PBLs)] from ten adult donors. In contrast to DDB2, TNFRSF10B and BAX were differentially modulated among the subpopulations and the PBLs, being more activated in irradiated CD19+ B and CD8+ T lymphocytes. The level of BAX activation in the various subpopulations correlated with the sensitivity of the cells to radiation, suggesting its possible role in the differential radiosensitivity of hematopoietic cell subsets.
KW - B, T, NK lymphocytes
KW - BAX, TNFRSF10B
KW - cDNA microarray
KW - Lymphocyte
KW - Quantitative real-time RT-PCR
KW - Radiation
KW - P53
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_27299
U2 - 10.1007/s00018-005-5086-3
DO - 10.1007/s00018-005-5086-3
M3 - Article
C2 - 15971001
SN - 1420-9071
VL - 62
SP - 1489
EP - 1501
JO - Cellular and Molecular Life Science
JF - Cellular and Molecular Life Science
IS - 13
ER -