In this paper, an experimental study is presented that intended to investigate (1) the anisotropy properties of hydraulic conductivity of BoomClay, (2) the effect of heating-cooling cycle on the hydraulic conductivity and intrinsic permeability of Boom Clay, and (3) the effect of loading-unloading cycle on the hydraulic conductivity and intrinsic permeability of Boom Clay. Constant-head tests were carried out in a temperature-controlled triaxial cell. First, the anisotropic characteristic of hydraulic conductivity of Boom Clay with respect to its bedding was confirmed. The horizontal hydraulic conductivity (parallel to bedding) is larger than the vertical hydraulic conductivity (perpendicular to bedding). Second, there was a positive and reversible relationship between the hydraulic conductivity and temperature and a negative and irreversible relationship between the hydraulic conductivity and hydrostatic pressure. Specifically, for both horizontal and vertical hydraulic conductivity, the value at 80 °C is approximately 2.4 times larger than that at room temperature (23 °C). However, it appears that the hydraulic conductivity is not sensitive to heating rate. Data analysis reveals that under variable temperature conditions, the changes in viscosity and density of water with temperature are the main factors affecting the change in hydraulic conductivity of Boom Clay with temperature, although other factors may have an effect to some extent.